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KMID : 0357919990330080545
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Korean Journal of Pathology
1999 Volume.33 No. 8 p.545 ~ p.554
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Expression of TGF-β and PDGF in Monocrotaline-Induced Pulmonary Hypertension in Rats
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Cho Min-Sun
Cho Sang-Ho
Yang Woo-Ick
Han Woon-Sup
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Abstract
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Pulmonary vascular hypertension is characterized by migration and proliferation of smooth muscle cells accompanying abnormal synthesis and accumulation of extracellular proteins in vascular wall. The aim of this study is to define the role of endogneous TGF-βs and PDGF in the process of remodeling vessels through determining the temporal and spatial distribution of these growth factors in hypertensive pulmonary vessels in monocrotaline-induced pulmonary hypertension in rat. Sprague-Dawley rats were sacrificed 12 hours, 1, 2, 4, 7, 10, 14, 21, 28, and 56 days after treatment. The morphometric analysis of medial thickening and immunohistochemical study using antibodies to TGF-β1, TGF-β2, TGF-β3, and PDGF were performed. Immunoreactivities for TGF-β1 and TGF-β3 were increased from the 14th day in the medial smooth muscle cells and PDGF showed increased expression from the 21st day in the medial smooth muscle cells. No difference in TGF-β2 immunoreactivity was found between control and experimental groups. The expression of TGF-β1, TGF-β3 and PDGF increased in medial layers with the progressive thickening of pulmonary arteries which was considered to have close relation to medial hypertrophy of pulmonary arterioles. In the case of PDGF, however, the morphologic change occurred before increase in immunoreactivity was observed in the medial layer of pulmonary arterioles. Moreover, the function of isoforms of TGF-β has yet to be completely elucidated; the different affinity to receptors and the degree of expression of these receptors that are supposed to affect the function of growth factors. Thus, further studies are needed.
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KEYWORD
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Pulmonary hypertension, Monocrotaline, TGF-β, PDGF, Immunohihtochemistry
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